Data-Independent Acquisition (DIA) metabolomics systematically and continuously fragments all ions within predefined mass isolation windows across the retention time range. Unlike traditional Data-Dependent Acquisition (DDA) that targets singular intense precursors, DIA records a comprehensive digital map of all fragment ions. While highly inclusive, this yields incredibly complex, multiplexed MS2 spectra.
This mzView DIA Deep Explorer uses a highly efficient Virtual File Indexing (VIF) architecture to seamlessly stream and query massive (8GB+) DIA mzML files. Web Workers dynamically locate and extract targeted scans directly from your local disk on-the-fly, preventing standard browser memory crashes.
Quick Start: Select your mzML file in Section 1. Once indexed, explore the MS1 Total Ion Chromatogram and Spectra in Section 2 to discover interesting mass features. Clicking any peak sets it as the active Target Precursor for Section 3. In Section 3, extract the MS2 Extracted Ion Chromatogram (EIC) for that window, select a target scan, and seamlessly query the IDSL Spectral Database to identify the precursor.
Example DIA Data files: ZenoTOF 8600 Metabolomics ZT Scan 3.0 DIA metabolomics dataset for NIST SRM 1950 plasma sample.